Classic Classic Quiz Are you an expert in DNA transfection? by Polyplus2011 Polyplus2011 134 played - 13 yrs ago Are you an expert in DNA transfection? Give the DNA transfection challenge a try! QUIZ 10 QUESTIONS hard 1 How do you check for DNA quality? Western blotSpectrophotometryCapillary cytometry (FACS) 2 Which A260/280 ratio will you consider good for DNA purity/quality? About 1. 3About 1. 8About 2. 3 3 Which cellular compartment should be reached by DNA to be expressed? NucleusMitochondriaCytoplasm 4 How many cellular barrier(s) should DNA cross to be expressed? 123 5 How many cell passages should you wait before performing transfection for optimal results? 0-55-20>30 6 Where should you prepare your DNA/transfection reagent complexes? Under the hoodOn the benchOn the Bench with a flame 7 Which method could be used to assess the transfection efficiency of a fluorescent reporter gene? SpectrophotometryCapillary cytometry (FACS)NMR spectroscopy 8 What is approximately the size of DNA/transfection reagent complexes for efficient transfection in plates? About 0. 5 picometerAbout 0. 5 nanometerAbout 0. 5 micrometer 9 Which plate size is commonly commercially available? 12-wells36-wells396-wells 10 How long does it takes after plasmid transfection for the encoded protein to be expressed? 5 min50 min5 h A mistake in this Quiz ? Contact the author Commentaires
